8U24

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	82.290, 82.290, 84.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.020 - 1.850
R-factor	0.1691
Rwork	0.168
R-free	0.19580
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.097	0.059	0.478
Total number of observations	178371
Number of reflections	25156	1413	1013
<I/σ(I)>	13
Completeness [%]	98.7	98.5	82
Redundancy	7.1	7.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Sodium acetate, 0.1 M Sodium cacodylate pH 6.5, 30% w/v PEG 8,000