8U23
A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.753, 145.623, 106.959 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 49.640 - 1.780 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.18530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.834 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.055 | 0.028 | 0.595 |
| Total number of observations | 768336 | ||
| Number of reflections | 206609 | 10373 | 10189 |
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 99.6 | 98.3 | 98.5 |
| Redundancy | 3.7 | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M SPG buffer pH 5, 25% w/v PEG 1500 |
