8U22

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97741
Spacegroup name	P 43 21 2
Unit cell lengths	54.582, 54.582, 169.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.630 - 1.800
R-factor	0.1826
Rwork	0.180
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.813
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.097	0.081	0.689
Rmeas		0.089	0.794
Rpim		0.037	0.387
Number of reflections	24574	1356	1150
<I/σ(I)>	10.4
Completeness [%]	98.7	94.7	94.7
Redundancy	5.8	5.5	4
CC(1/2)		0.990	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	50mM NaBr, 25% PEG3350