8U20

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	50.357, 87.588, 147.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.660 - 1.900
R-factor	0.1744
Rwork	0.173
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.768
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.063	0.033	0.372
Total number of observations	185682
Number of reflections	49669	2827	2227
<I/σ(I)>	14.4
Completeness [%]	95.3	99.2	86.5
Redundancy	3.7	4.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M NH4Cl, 0.1M Sodium acetate pH 5, 20% w/v PEG 6000