8U1Z

Crystal structure of the Fis1 cytosolic domain bound to a peptide inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	54.798, 54.798, 103.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.570 - 1.850
R-factor	0.2131
Rwork	0.208
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nzn
RMSD bond length	0.006
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.400	48.400	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.063	0.028	2.135
Rmeas	0.068	0.030	2.352
Number of reflections	14066	320	1813
<I/σ(I)>	15.85
Completeness [%]	99.0
Redundancy	13.7
CC(1/2)	0.999	0.999	0.202

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	PEG3350, HEPES, sodium chloride, Silver Bullet D5 (Hampton Research HR2-996-41)