8U0X

Yeast Pex6 N1(1-184) Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1158
Spacegroup name	P 3 2 1
Unit cell lengths	85.317, 85.317, 51.192
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.890 - 1.860
R-factor	0.2142
Rwork	0.212
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.999
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.890	73.887	1.895
High resolution limit [Å]	1.860	5.056	1.863
Rmerge	0.088	0.033	1.361
Rmeas	0.091	0.034	1.411
Rpim	0.024	0.009	0.369
Number of reflections	18315	990	927
<I/σ(I)>	20.2	59.4	2.3
Completeness [%]	99.9	99.8	100
Redundancy	1
CC(1/2)	0.999	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	0.8 M LiCl, 0.1 M citric acid pH 5, 16% PEG6000