8U03

Crystal structure of non-glycosylated 10E8-GT10.1 scaffold in complex with a human 10E8 NGS precursor (10E8-NGS-03)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	69.599, 85.218, 175.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.400 - 2.720
R-factor	0.2212
Rwork	0.220
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.760
High resolution limit [Å]	2.720	2.720
Rpim	0.037	0.530
Number of reflections	27880	1065
<I/σ(I)>	19.4	1.2
Completeness [%]	96.2	73.8
Redundancy	6	4.2
CC(1/2)	0.970	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Imidazole pH 8, 40% Peg400