8TZN

Crystal structure of 10E8-GT10.2 HIV-1 MPER scaffold in complex with a non-human primate W3-01 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 32 2 1
Unit cell lengths	111.825, 111.825, 199.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.150 - 3.110
R-factor	0.2482
Rwork	0.246
R-free	0.28360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.218
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5058)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.170
High resolution limit [Å]	3.110	3.110
Number of reflections	26452	2590
<I/σ(I)>	16.7
Completeness [%]	100.0
Redundancy	17.7
CC(1/2)	1.000	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293.15	0.2M ammonium dihydrogen phosphate, 20% PEG3350, and 10% (v/v) ethylene glycol