8TZ0
Structure of a bacterial E1-E2-Ubl complex (form 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.851, 89.659, 131.601 |
Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
Resolution | 74.010 - 2.470 |
R-factor | 0.1986 |
Rwork | 0.196 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.660 | 2.530 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.093 | 0.934 |
Number of reflections | 60824 | 4283 |
<I/σ(I)> | 11.9 | 1.5 |
Completeness [%] | 98.9 | 95 |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM HEPES pH 7.5, 100 mM sodium citrate, 5% isopropanol, and 10% PEG 3350 |