8TYY

Structure of a bacterial Ubl-deubiquitinase complex (form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	F 4 3 2
Unit cell lengths	214.891, 214.891, 214.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 1.680
R-factor	0.1477
Rwork	0.147
R-free	0.16280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	124.070	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.070	0.956
Number of reflections	48723	2454
<I/σ(I)>	35.8	5.2
Completeness [%]	99.9
Redundancy	40.3
CC(1/2)	1.000	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM MES pH 6.5 and 1M sodium/potassium tartrate