8TYX

Structure of a bacterial Ubl-deubiquitinase complex (form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	53.338, 64.690, 141.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.850 - 1.360
R-factor	0.1548
Rwork	0.153
R-free	0.17990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.941
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.850	1.380
High resolution limit [Å]	1.360	1.360
Rmerge	0.058	0.481
Number of reflections	53040	2551
<I/σ(I)>	25	4
Completeness [%]	99.9
Redundancy	12.7
CC(1/2)	0.999	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM HEPES pH 7.5, 0.2 M MgCl2, and 25% PEG 3350