8TYS
Adaptive mechanism of collagen IV scaffold assembly in Drosophila: crystal structure of tissue-extracted NC1 hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-03-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 63 |
| Unit cell lengths | 145.209, 145.209, 106.491 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.630 - 2.900 |
| R-factor | 0.2428 |
| Rwork | 0.241 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.521 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.630 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.085 | 0.450 |
| Rmeas | 0.093 | 0.490 |
| Rpim | 0.039 | 0.200 |
| Number of reflections | 28410 | 4562 |
| <I/σ(I)> | 17.3 | 4.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 35% MPD, 0.1 M NaCl, 100mM HEPES pH 7.5 |
