Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.772, 86.994, 192.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.640 - 1.640 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.343 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 1.185 | |
| Rpim | 0.471 | |
| Number of reflections | 65130 | 3146 |
| <I/σ(I)> | 14.9 | 1.7 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 6.8 | 7.2 |
| CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.1M SPG 25% PEG 1500 12% Inosotol 0.2mM DTT 1mM TEW |
