8TXP

Crystal structure of 05.GC.w13.01 Fab in complex with H1 HA from A/California/04/2009(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 3 2 1
Unit cell lengths	128.350, 128.350, 155.756
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.240 - 2.750
R-factor	0.2517
Rwork	0.250
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m4y
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	2.800
High resolution limit [Å]	2.750	2.760
Rpim	0.035	0.423
Number of reflections	38915	1962
<I/σ(I)>	21.6	1.3
Completeness [%]	100.0	100
Redundancy	16.2
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate, pH 6.2, 20% PEG3350