8TXM

Crystal structure of 05.GC.w13.02 Fab in complex with H1 HA from A/California/04/2009(H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97934
Spacegroup name	P 21 3
Unit cell lengths	167.531, 167.531, 167.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 3.250
R-factor	0.2759
Rwork	0.275
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m4y
RMSD bond length	0.002
RMSD bond angle	0.441
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	3.310
High resolution limit [Å]	3.250	3.250
Rpim		0.053
Number of reflections	24917	1320
<I/σ(I)>	12
Completeness [%]	100.0	100
Redundancy	13.6
CC(1/2)	1.000	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium acetate, pH 6.2, 20% PEG3350