8TX6

Crystal structure of an engineered variant of galactose oxidase, GOaseRd7BB, from Fusarium graminearum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	97.623, 88.920, 86.167
Unit cell angles	90.00, 117.82, 90.00

Refinement procedure

Resolution	22.750 - 1.560
R-factor	0.1654
Rwork	0.164
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.158
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	BUSTER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.750	1.850
High resolution limit [Å]	1.560	1.760
Number of reflections	79190	79190
<I/σ(I)>	15
Completeness [%]	85.3
Redundancy	3.4
CC(1/2)	0.999	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	20% vol PEG 3350, 4% vol Tacsimate, 100 mM acetate buffer (pH 4.6)