8TW1
Crystal structure of Lys2972, a phage endolysin targeting Streptococcus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.180, 64.792, 55.426 |
| Unit cell angles | 90.00, 122.68, 90.00 |
Refinement procedure
| Resolution | 28.500 - 1.270 |
| R-factor | 0.16968 |
| Rwork | 0.169 |
| R-free | 0.19083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.938 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.340 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmeas | 0.043 | 0.671 |
| Number of reflections | 63597 | 9345 |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 97.6 | 98.4 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 295 | 0.2 M lithium chloride, 0.1 M sodium acetate pH 5.0, 20% PEG-6000 |
