8TUV

Merkel Cell Polyomavirus LTA NLSm bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.273, 89.516, 97.221
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.890 - 2.300
R-factor	0.1929
Rwork	0.191
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.509
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.890	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.130	1.060
Rmeas	0.140	1.140
Rpim	0.050	0.420
Number of reflections	30660	2946
<I/σ(I)>	8.5	2
Completeness [%]	100.0
Redundancy	7.5
CC(1/2)	1.000	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.75 M sodium citrate, 0.1 M HEPES pH 6.5, 0.01 M DTT