8TTD

Structure of VPS29 complexed with Fam21A repeat 21 (1328-1341)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95365
Spacegroup name	P 32 2 1
Unit cell lengths	43.342, 43.342, 165.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.602 - 2.011
R-factor	0.1873
Rwork	0.184
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.280	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.052	0.475
Rmeas	0.053	0.488
Rpim	0.013	0.110
Total number of observations	239643	17499
Number of reflections	12765	911
<I/σ(I)>	25.4	4.5
Completeness [%]	99.9
Redundancy	18.8	19.2
CC(1/2)	1.000	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Magnesium Formate, 20% PEG3350