8TT2
Pseudomonas fluorescens isocyanide hydratase pH=5.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.886 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.532, 56.790, 68.769 |
| Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
| Resolution | 38.450 - 1.330 |
| R-factor | 0.1472 |
| Rwork | 0.146 |
| R-free | 0.17630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.856 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.450 | 1.360 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmeas | 0.067 | 2.504 |
| Number of reflections | 89288 | 4619 |
| <I/σ(I)> | 13.3 | 0.8 |
| Completeness [%] | 97.3 | 95.8 |
| Redundancy | 6.1 | 6.1 |
| CC(1/2) | 0.999 | 0.325 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 23% PEG 3350, 200 mM MgCl2, 2 mM DTT and 100mM sodium acetate pH=5.4 |
