8TSY
Pseudomonas fluorescens G150T-2 isocyanide hydratase at 274 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 274 |
Detector technology | PIXEL |
Collection date | 2018-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.775 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 72.202, 59.725, 56.373 |
Unit cell angles | 90.00, 115.88, 90.00 |
Refinement procedure
Resolution | 35.210 - 1.200 |
R-factor | 0.1185 |
Rwork | 0.117 |
R-free | 0.13710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data reduction software | autoXDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.210 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmeas | 0.078 | 2.243 |
Number of reflections | 66132 | 3293 |
<I/σ(I)> | 7.3 | 1.1 |
Completeness [%] | 98.3 | 95.8 |
Redundancy | 3.8 | 3.5 |
CC(1/2) | 0.997 | 0.334 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 23% polyethylene glycol (PEG) 3350, 100mM Tris-HCl, pH 8.6, 200mM magnesium chloride, and 2mM dithiothreitol |