8TSV
Crystal structure of the Zika virus stem-loop A (SLA) top stem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-12-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.226, 52.532, 53.670 |
| Unit cell angles | 63.60, 88.08, 85.94 |
Refinement procedure
| Resolution | 29.150 - 1.510 |
| R-factor | 0.1563 |
| Rwork | 0.155 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.150 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.034 | 0.144 |
| Rmeas | 0.040 | 0.174 |
| Rpim | 0.020 | 0.096 |
| Number of reflections | 43189 | 2043 |
| <I/σ(I)> | 39 | |
| Completeness [%] | 96.4 | 90.2 |
| Redundancy | 3.9 | 3.2 |
| CC(1/2) | 0.995 | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Sodium Acetate, Magnesium Sulfate, Lithium Sulfate, Isopropanol |
