8TSV

Crystal structure of the Zika virus stem-loop A (SLA) top stem

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	29.226, 52.532, 53.670
Unit cell angles	63.60, 88.08, 85.94

Refinement procedure

Resolution	29.150 - 1.510
R-factor	0.1563
Rwork	0.155
R-free	0.17500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.351
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.150	1.540
High resolution limit [Å]	1.510	1.510
Rmerge	0.034	0.144
Rmeas	0.040	0.174
Rpim	0.020	0.096
Number of reflections	43189	2043
<I/σ(I)>	39
Completeness [%]	96.4	90.2
Redundancy	3.9	3.2
CC(1/2)	0.995	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Sodium Acetate, Magnesium Sulfate, Lithium Sulfate, Isopropanol