8TSM

Crystal structure of chicken Netrin-1 LN LE1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	I 41 2 2
Unit cell lengths	112.413, 112.413, 242.644
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.770 - 3.900
R-factor	0.3124
Rwork	0.312
R-free	0.31910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.20.1-4487_4487)
Refinement software	PHENIX (1.20.1-4487_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.770	4.040
High resolution limit [Å]	3.900	3.900
Rpim	0.070	1.268
Number of reflections	13196	1682
<I/σ(I)>	7	0.87
Completeness [%]	97.8	96.8
Redundancy	13.1	13.8
CC(1/2)	0.999	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium phosphate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350