8TP8

Structure of the C. crescentus WYL-activator, DriD, bound to ssDNA and cognate DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.260, 178.941, 92.150
Unit cell angles	90.00, 95.24, 90.00

Refinement procedure

Resolution	68.970 - 2.740
R-factor	0.1975
Rwork	0.196
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.000	2.890
High resolution limit [Å]	2.740	2.740
Rmerge	0.092	0.657
Rpim	0.060
Number of reflections	56088	3276
<I/σ(I)>	7.6	1.5
Completeness [%]	96.1
Redundancy	3.3
CC(1/2)	0.990	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris, 1.0 M Citrate