8TON

Crystal Structure of Engineered Mouse Protocadherin-15 EC3-EC7 Connection

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918 A
Spacegroup name	P 32 2 1
Unit cell lengths	84.603, 84.603, 76.895
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.340 - 1.940
Rwork	0.184
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.713
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.940	1.940
Number of reflections	23952	1173
<I/σ(I)>	31.9285	1.86
Completeness [%]	99.9	100
Redundancy	11.6	10.4
CC(1/2)	1.000	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	15% w/v PEG 20000, 0.1 M Tris HCl pH 8.5, 0.6 uM Spermine Tetrahydrochloride