8TNN

Crystal structure of Epstein-Barr virus gH/gL/gp42 in complex with gp42 antibody A10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	88.750, 138.960, 316.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.830 - 3.360
R-factor	0.236
Rwork	0.234
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.770
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.670	3.410
High resolution limit [Å]	3.360	3.360
Number of reflections	56676	5565
<I/σ(I)>	7.3
Completeness [%]	99.9
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	278	20mM Tris pH7.5, 150mM NaCl, 0.1M citric-acid-BIS-TRIS propane, and 10% PEG 3,350