8TN5

The Crystal Structure of a human monoclonal antibody (aAb), termed TG10, complexed with a GlcNH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	90.524, 90.524, 207.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.400 - 1.760
R-factor	0.16482
Rwork	0.164
R-free	0.20622
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.569
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.400	1.800
High resolution limit [Å]	1.760	1.760
Rmerge	0.072	0.632
Number of reflections	49758	9073
<I/σ(I)>	28.1
Completeness [%]	98.2
Redundancy	18.3
CC(1/2)	1.000	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.4M Ammonium Sulfate