8TMV

HTLV-1 capsid protein N-terminal domain triclinic crystal form with phosphate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	28.195, 30.720, 36.081
Unit cell angles	65.19, 79.30, 89.56

Refinement procedure

Resolution	27.800 - 1.730
R-factor	0.2015
Rwork	0.200
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ere
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.080	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.067	1.007
Rmeas	0.079	1.203
Rpim	0.042	0.648
Total number of observations	43879	1657
Number of reflections	12672	532
<I/σ(I)>	6.2	0.7
Completeness [%]	95.9
Redundancy	3.5	3.1
CC(1/2)	0.998	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% ethylene glycol, 10% PEG 8K, 0.1 M HEPES, pH 7.5