8TLL

CDCA7 (Mouse) Binds Non-B-form 26-mer DNA oligo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	87.710, 60.724, 84.106
Unit cell angles	90.00, 113.96, 90.00

Refinement procedure

Resolution	48.400 - 2.580
R-factor	0.2374
Rwork	0.235
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	2.680
High resolution limit [Å]	2.580	2.580
Rmerge	0.199	0.609
Rpim	0.072	0.444
Number of reflections	12207	814
<I/σ(I)>	8.7	1.6
Completeness [%]	93.6
Redundancy	7	1.9
CC(1/2)	0.967	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M MgCl2, 0.1 M Tris pH 8.5, 23%PEG3350