8TLJ

CDCA7 (Mouse) Binds Non-B-form 32-mer DNA oligo Containing a 5mC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	169.874, 31.533, 91.823
Unit cell angles	90.00, 110.75, 90.00

Refinement procedure

Resolution	45.030 - 2.300
R-factor	0.1868
Rwork	0.185
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.030	2.370
High resolution limit [Å]	2.300	2.300
Rmerge	0.061	0.419
Rpim	0.038	0.321
Number of reflections	19726	1408
<I/σ(I)>	16.7	1.6
Completeness [%]	95.1	69
Redundancy	3.1	1.9
CC(1/2)	0.997	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M MgCl2, 0.1 M Bis-Tris pH 6.2, 25% PGE3350