8TLI

THERMOLYSIN (100% ISOPROPANOL SOAKED CRYSTALS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-05-01
Detector	MAR scanner 300 mm plate
Spacegroup name	P 61 2 2
Unit cell lengths	93.040, 93.040, 130.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.171
R-free	0.22300
Structure solution method	OTHER
RMSD bond length	0.016
RMSD bond angle	0.041
Data reduction software	DENZO
Data scaling software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.350
High resolution limit [Å]	2.200	2.200
Rmerge	0.108	0.363
Number of reflections	16875
<I/σ(I)>	5.4	2.1
Completeness [%]	97.0	95
Redundancy	5.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.50