8TLH

CDCA7 (Mouse) Binds Non-B-form 32-mer DNA oligo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	169.679, 31.523, 91.634
Unit cell angles	90.00, 110.59, 90.00

Refinement procedure

Resolution	39.710 - 1.940
R-factor	0.2005
Rwork	0.199
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.536
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.710	2.020
High resolution limit [Å]	1.940	1.940
Rmerge	0.080	0.678
Rpim	0.035	0.464
Number of reflections	29047	1162
<I/σ(I)>	17.8	1.1
Completeness [%]	84.8
Redundancy	5.2
CC(1/2)	0.997	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1 M MgCl2, 0.1 M Tris pH8.5 and 25 % PEG3350.