8TLG

CDCA7 (Mouse) Binds Non-B-form 34-mer DNA oligo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	86.491, 60.562, 53.229
Unit cell angles	90.00, 123.10, 90.00

Refinement procedure

Resolution	30.280 - 2.090
R-factor	0.1874
Rwork	0.186
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.280	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.068	0.342
Rpim	0.037	0.257
Number of reflections	11460	653
<I/σ(I)>	15.5	2.8
Completeness [%]	83.6
Redundancy	3.6
CC(1/2)	1.000	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.1 M MgCl2, 0.1 M Tris pH8.5 and 25 % PEG3350.