8TLF

CDCA7 (Mouse) Binds Non-B-form DNA oligo 36-mer (sg C2-Form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	89.400, 31.492, 133.324
Unit cell angles	90.00, 95.75, 90.00

Refinement procedure

Resolution	29.900 - 1.640
R-factor	0.1929
Rwork	0.192
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	1.710
High resolution limit [Å]	1.640	1.640
Rmerge	0.095	0.509
Rpim	0.049	0.343
Number of reflections	72067	4182
<I/σ(I)>	13.6	2.2
Completeness [%]	89.9	65.4
Redundancy	3.9	2.1
CC(1/2)	0.992	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.2 M MgCl2, 0.1 M Tris pH8.5, 25%PEG3350