8TLE

CDCA7 (Mouse) Binds Non-B-form 36-mer DNA oligo (sg C2-Form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	88.666, 60.768, 49.090
Unit cell angles	90.00, 117.51, 90.00

Refinement procedure

Resolution	30.380 - 2.080
R-factor	0.2335
Rwork	0.232
R-free	0.26190
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.502
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.380	2.180
High resolution limit [Å]	2.080	2.080
Rmerge	0.165	0.490
Rpim	0.047	0.178
Number of reflections	25246	1147
<I/σ(I)>	13.1	3.7
Completeness [%]	97.2	85.6
Redundancy	5.9	3.3
CC(1/2)	0.990	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	0.2 M MgCl2, 0.1 M Tris pH8.5, 25%PEG3350