8TKN

Murine NF-kappaB p50 Rel Homology Region homodimer in complex with 10-mer kappaB DNA from human Neutrophil Gelatinase-associated Lipocalin (NGAL) promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	67.006, 102.644, 127.704
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 2.800
R-factor	0.2413
Rwork	0.239
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nfk
RMSD bond length	0.004
RMSD bond angle	0.585
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	23123	2310
<I/σ(I)>	19	2.3
Completeness [%]	100.0
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	0.05 M sodium acetate pH 5.4, 20 mM magnesium sulfate, and 4% PEG 3350