8TJJ
SAM-dependent methyltransferase RedM bound to SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.860, 58.780, 113.920 |
| Unit cell angles | 90.00, 103.11, 90.00 |
Refinement procedure
| Resolution | 45.640 - 1.820 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.766 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.200 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.146 | 1.215 |
| Rmeas | 0.159 | 1.316 |
| Rpim | 0.061 | 0.501 |
| Number of reflections | 125933 | 6228 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 95.9 | 96.7 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.997 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2-0.3 M ammonium sulphate, 0.1 M MES pH 6.5, 18-24 % PEG MME 5000, 13-16 mg.mL RedM |
