8TJB

CRYSTAL STRUCTURE OF THE A/Texas/73/2017(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03317
Spacegroup name	H 3 2
Unit cell lengths	100.240, 100.240, 391.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.140 - 2.450
R-factor	0.2424
Rwork	0.238
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.607
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.140	2.550
High resolution limit [Å]	2.450	2.450
Rpim	0.040	0.410
Number of reflections	28518	3105
<I/σ(I)>	30	2.2
Completeness [%]	99.9
Redundancy	9.1
CC(1/2)	0.998	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	30% 1,2-propanediol, 20% PEG-400, and 0.1M HEPES pH 7.5