8TJ7

CRYSTAL STRUCTURE OF THE A/Shandong/9/1993(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	H 3
Unit cell lengths	98.329, 98.329, 270.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.250 - 2.850
R-factor	0.2508
Rwork	0.245
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.154
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.250	2.950
High resolution limit [Å]	2.850	2.850
Rpim	0.040	0.520
Number of reflections	22883	2316
<I/σ(I)>	8.6	1.2
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)	0.967	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% PEG-400 and 0.1 M imidazole pH 8.0