8THS
Crystal Structure of a reconstructed Kaede-type Red Fluorescent Protein, LEA A69T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.447, 106.084, 123.471 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.030 - 1.500 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.18806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.769 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.066 | 1.814 |
| Rmeas | 0.071 | 2.050 |
| Rpim | 0.025 | 0.927 |
| Total number of observations | 1183642 | 33660 |
| Number of reflections | 152026 | 7274 |
| <I/σ(I)> | 18.3 | 0.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.8 | 4.6 |
| CC(1/2) | 1.000 | 0.331 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2 microliters of protein at 20 mg/mL in 10 mM HEPES pH 7.5 + 75 mM NaCl mixed with 2 microliters of well solution containing 35% PEG 3350 + 0.3 M potassium nitrate |
