8THM
Beta carbonic anhydrase from the carboxysome of Cyanobium PCC 7001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.314, 181.848, 190.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.510 - 2.300 |
| R-factor | 0.1958 |
| Rwork | 0.193 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.260 | 5.093 |
| Rmeas | 0.271 | 5.302 |
| Rpim | 0.077 | 1.468 |
| Total number of observations | 1970240 | 100213 |
| Number of reflections | 160617 | 7868 |
| <I/σ(I)> | 8.1 | 0.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 | 12.7 |
| CC(1/2) | 0.997 | 0.297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2M Ammonium sulfate, 0.1M BIS-TRIS (pH 6.5), 21% PEG 3350, 15% Ethylene glycol |
