8THA

1TEL, non-compressed, double-helical crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 64
Unit cell lengths	69.163, 69.163, 29.131
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.950 - 1.680
Rwork	0.182
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.124
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.950	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.080	1.521
Rmeas	0.082	1.573
Rpim	0.020	0.391
Number of reflections	8707	613
<I/σ(I)>	25.74	2.01
Completeness [%]	93.8
Redundancy	17.1
CC(1/2)	1.000	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.5 M Bis-Tris, 0.35 M Mg-Formate, 5 mg/mL protein