8TH7
Crystal Structure of the G3BP1 NTF2-like domain bound to the Caprin1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.650, 71.440, 51.950 |
| Unit cell angles | 90.00, 111.26, 90.00 |
Refinement procedure
| Resolution | 48.410 - 2.880 |
| R-factor | 0.19448 |
| Rwork | 0.190 |
| R-free | 0.27652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q9o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.420 | 2.955 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Number of reflections | 6159 | 966 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.938 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291.15 | 1.8M tri-ammonium citrate pH 7.0 |
