8TGC

Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979310
Spacegroup name	P 21 21 21
Unit cell lengths	37.596, 67.895, 71.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.660 - 1.484
Rwork	0.203
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.437
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.4.03)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.660	1.643
High resolution limit [Å]	1.484	1.484
Rmerge	0.074	0.699
Rmeas	0.082	0.853
Rpim	0.033	0.475
Number of reflections	19557	978
<I/σ(I)>	13.4	1.4
Completeness [%]	63.3	12.2
Redundancy	5.6	3.1
CC(1/2)	0.999	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	2.4 M ammonium phosphate dibasic, 0.1M Tris pH 8.5