8TG8

Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	70.042, 70.042, 38.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.527 - 1.576
Rwork	0.201
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.632
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.4.03)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.527	1.683
High resolution limit [Å]	1.576	1.576
Rmerge	0.054	2.123
Rmeas	0.056	2.213
Rpim	0.016	0.615
Number of reflections	11588	580
<I/σ(I)>	18.8	1.2
Completeness [%]	84.6	24.3
Redundancy	12.3	11.8
CC(1/2)	1.000	0.473

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	1.2 M sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.6