8TFT
Fab of O13-1 human IgG1 antibody bound to IgV domain of human TIM-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.338, 69.785, 93.632 |
| Unit cell angles | 90.00, 99.90, 90.00 |
Refinement procedure
| Resolution | 37.003 - 2.300 |
| Rwork | 0.244 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.964 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.065 | |
| Number of reflections | 69615 | 436 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.930 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 MIB buffer (Sodium malonate dibasic monohydrate, Imidazole, Boric acid), pH 6.0 and 25% w/v PEG 1500 |
