8TFI

tRNA 2'-phosphotransferase (Tpt1) from Pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	38.464, 44.807, 124.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.170 - 1.970
R-factor	0.1948
Rwork	0.193
R-free	0.22820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.810	2.020
High resolution limit [Å]	1.970	1.970
Rmerge	0.067	0.839
Rpim	0.025	0.297
Number of reflections	15955	1096
<I/σ(I)>	16.5	2
Completeness [%]	100.0	100
Redundancy	8.4	8.8
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1-0.3 M tris-bicine, 0.1 M amino acid additives mixture (0.02 M each of DL-glutamic acid monohydrate, DL-alanine, glycine, DL-lysine monohydrate and DL-serine), 16-29.6% ethylene glycol, 8-14.8% PEG-8000