8TE7
Structure of TRNM-f.01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 136.895, 136.895, 94.903 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.810 - 3.180 |
| R-factor | 0.2354 |
| Rwork | 0.233 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.005 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.124 | |
| Rmeas | 0.134 | |
| Number of reflections | 17587 | 844 |
| <I/σ(I)> | 19.45 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7 | |
| CC(1/2) | 0.988 | 0.824 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M LiSO4 1.3 M NaH2PO4 0.8 M KH2PO4 0.1 M CAPS pH 10.5 |
