8TE7
Structure of TRNM-f.01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 136.895, 136.895, 94.903 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.810 - 3.180 |
R-factor | 0.2354 |
Rwork | 0.233 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.180 | 3.180 |
Rmerge | 0.124 | |
Rmeas | 0.134 | |
Number of reflections | 17587 | 844 |
<I/σ(I)> | 19.45 | |
Completeness [%] | 99.7 | |
Redundancy | 7 | |
CC(1/2) | 0.988 | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M LiSO4 1.3 M NaH2PO4 0.8 M KH2PO4 0.1 M CAPS pH 10.5 |