8TE0
16mer self-complementary duplex RNA with D:C pair sequence 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2023-06-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033216 |
Spacegroup name | H 3 2 |
Unit cell lengths | 42.501, 42.501, 129.967 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.414 - 1.420 |
Rwork | 0.215 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 2.370 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.065 | 0.268 |
Rmeas | 0.069 | 0.345 |
Rpim | 0.024 | 0.214 |
Number of reflections | 8586 | 267 |
<I/σ(I)> | 30 | 2.8 |
Completeness [%] | 96.2 | 64.5 |
Redundancy | 7.1 | 2 |
CC(1/2) | 0.983 | 0.900 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10 mM Magnesium chloride hexahydrate, 50 mM HEPES sodium pH 7.0, 4.0 M Lithium chloride |