8TE0

16mer self-complementary duplex RNA with D:C pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-06-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033216
Spacegroup name	H 3 2
Unit cell lengths	42.501, 42.501, 129.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.414 - 1.420
Rwork	0.215
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.370
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.065	0.268
Rmeas	0.069	0.345
Rpim	0.024	0.214
Number of reflections	8586	267
<I/σ(I)>	30	2.8
Completeness [%]	96.2	64.5
Redundancy	7.1	2
CC(1/2)	0.983	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	10 mM Magnesium chloride hexahydrate, 50 mM HEPES sodium pH 7.0, 4.0 M Lithium chloride