8TDX

TRNM-b.01 in complex with HIV Env fusion peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	84.464, 88.926, 129.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 2.090
R-factor	0.2057
Rwork	0.204
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.733
Data reduction software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.103	0.624
Rmeas	0.113
Number of reflections	57876	2853
<I/σ(I)>	14.48	2.08
Completeness [%]	99.5
Redundancy	5.9
CC(1/2)	0.996	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	0.2M Sodium Chloride 21% PEG 8K 0.1M Sodium Phosphate / Citric Acid pH 4.2